Serological tests: Detection of antibodies in serum by enzyme-linked immunosorbent assay
ثبت نشده
چکیده
mastitis, arthritis, keratoconjunctivitis and, occasionally, abortion. Mycoplasma agalactiae (Ma) is the main cause of the disease in sheep and goats, but M. capricolum subsp. capricolum (Mcc), M. mycoides subsp. capri (Mmc) and M. putrefaciens produce a clinically similar disease, more often in goats, which may be accompanied by pneumonia. Ma and Mcc have been isolated from wild small ruminants such as ibex and mountain goats. Antibodies to Mmc and Mcc have been detected in South American camelids (alpacas, llamas and vicunas), but no mycoplasmas have yet been isolated.
منابع مشابه
Enzyme-Linked Immunosorbent Assay of Progesterone in Serum Using Penicillinase as Label
An enzyme-linked immunosorbent assay (ELISA) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. A C3 and C11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (BSA). Polyclonal antibody against these two immunogens were prepared in New Zealand white rabbits. Purified Ig fractions of antibodies were immobilized o...
متن کاملDesigning an enzyme-linked immunosorbent assay for detection of anti-penicillin antibodies levels in various species of animals in Khuzestan province, Iran
The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals...
متن کاملMolecular Evidence of Chlamydia trachomatis Infection and its Relation to Miscarriage
Objective Chlamydia trachomatis (CT) infection is the most common sexually transmitted disease in the world that can persist and also ascend in the genital tract. This intracellular and silent infection is related to some adverse pregnancy outcomes, such as miscarriage. The aims of this study were to explore the best CT screening tests using blood and vaginal samples and to investigate the corr...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملAdaptation of an indirect enzyme-linked immunosorbent assay by purified gp51SU for detection of antibodies to bovine leukemia virus
The objective of this study was to compare an indirect ELISA, based on a purified 60 kDa envelope glycoprotein (gp51SU), with a Pourquire indirect ELISA for the detection of antibodies to the bovine leukemia virus. For conducting this research, 340 serum samples were collected from two different breeds of cows (Sarabi and Holestin) in different herds. Commercial ELISA revealed positive results ...
متن کاملEvaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (BHV-1) antibodies in cattle
To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were col...
متن کامل